| | FIXTURE | VENUE | GMT | LOCAL | POOL |
| | Fri, 2 October 2020 |
| | Cardiff Blues v Zebre | Stadio Sergio Lanfranchi | 17:00 | 19:00 | Round 1 |
| | Gwent Dragons v Leinster | RDS Arena | 19:15 | 21:15 | Round 1 |
| | Benetton Treviso v Ulster | Kingspan Stadium | 19:15 | 21:15 | Round 1 |
| |
| | Sat, 3 October 2020 |
| | Munster v Scarlets | Parc y Scarlets | 14:00 | 16:00 | Round 1 |
| | Connacht v Glasgow Warriors | The Sportsground | 16:15 | 18:15 | Round 1 |
| | Edinburgh v Ospreys | BT Murrayfield Stadium | 18:35 | 20:35 | Round 1 |
| |
| | Fri, 9 October 2020 |
| | Gwent Dragons v Zebre | Rodney Parade | 19:15 | 21:15 | Round 2 |
| |
| | Sat, 10 October 2020 |
| | Benetton Treviso v Leinster | Stadio Comunale Di Monigo | 16:15 | 18:15 | Round 2 |
| | Ospreys v Ulster | Liberty Stadium | 16:15 | 18:15 | Round 2 |
| | Cardiff Blues v Connacht | Cardiff Arms Park | 18:35 | 20:35 | Round 2 |
| | Edinburgh v Munster | Thomond Park | 18:35 | 20:35 | Round 2 |
| |
| | Sun, 11 October 2020 |
| | Glasgow Warriors v Scarlets | Scotstoun Stadium | 16:15 | 18:15 | Round 2 |
| |
| | Fri, 23 October 2020 |
| | Leinster v Zebre | RDS Arena | 18:35 | 20:35 | Round 3 |
| | Benetton Treviso v Scarlets | Stadio Comunale Di Monigo | 19:15 | 21:15 | Round 3 |
| |
| | Sat, 24 October 2020 |
| | Glasgow Warriors v Ospreys | Liberty Stadium | 16:30 | 18:30 | Round 3 |
| |
| | Sun, 25 October 2020 |
| | Gwent Dragons v Ulster | Kingspan Stadium | 15:00 | 17:00 | Round 3 |
| | Connacht v Edinburgh | BT Murrayfield Stadium | 19:35 | 21:35 | Round 3 |
| |
| | Mon, 26 October 2020 |
| | Cardiff Blues v Munster | Thomond Park | 20:15 | 22:15 | Round 3 |
| |
| | Sun, 1 November 2020 |
| | Edinburgh v Scarlets | Parc y Scarlets | 18:45 | 20:45 | Round 4 |
| |
| | Mon, 2 November 2020 |
| | Cardiff Blues v Ulster | Cardiff Arms Park | 18:00 | 20:00 | Round 4 |
| | Ospreys v Zebre | Stadio Sergio Lanfranchi | 19:15 | 21:15 | Round 4 |
| | Glasgow Warriors v Leinster | Scotstoun Stadium | 20:15 | 22:15 | Round 4 |
| |
| | Sun, 8 November 2020 |
| | Leinster v Ospreys | Liberty Stadium | 15:00 | 17:00 | Round 5 |
| | Scarlets v Zebre | Parc y Scarlets | 17:15 | 19:15 | Round 5 |
| |
| | Mon, 9 November 2020 |
| | Cardiff Blues v Edinburgh | BT Murrayfield Stadium | 19:45 | 21:45 | Round 5 |
| | Glasgow Warriors v Ulster | Kingspan Stadium | 20:15 | 22:15 | Round 5 |
| |
| | Sat, 14 November 2020 |
| | Connacht v Scarlets | The Sportsground | 19:35 | 21:35 | Round 6 |
| |
| | Sun, 15 November 2020 |
| | Munster v Ospreys | Thomond Park | 14:45 | 16:45 | Round 6 |
| |
| | Mon, 16 November 2020 |
| | Ulster v Zebre | Stadio Sergio Lanfranchi | 18:00 | 20:00 | Round 6 |
| | Benetton Treviso v Cardiff Blues | Cardiff Arms Park | 19:45 | 21:45 | Round 6 |
| | Edinburgh v Leinster | RDS Arena | 20:15 | 22:15 | Round 6 |
| |
| | Sun, 22 November 2020 |
| | Benetton Treviso v Ospreys | Liberty Stadium | 13:45 | 15:45 | Round 7 |
| | Connacht v Zebre | Stadio Sergio Lanfranchi | 14:30 | 16:30 | Round 7 |
| | Cardiff Blues v Leinster | RDS Arena | 17:15 | 19:15 | Round 7 |
| | Scarlets v Ulster | Kingspan Stadium | 19:35 | 21:35 | Round 7 |
| |
| | Mon, 23 November 2020 |
| | Glasgow Warriors v Munster | Scotstoun Stadium | 20:15 | 22:15 | Round 7 |
| |
| | Sun, 29 November 2020 |
| | Benetton Treviso v Gwent Dragons | Stadio Comunale Di Monigo | 16:00 | 18:00 | Round 8 |
| | Cardiff Blues v Glasgow Warriors | Cardiff Arms Park | 19:35 | 21:35 | Round 8 |
| |
| | Mon, 30 November 2020 |
| | Munster v Zebre | Thomond Park | 20:15 | 22:15 | Round 8 |
| | Edinburgh v Ulster | BT Murrayfield Stadium | 20:15 | 22:15 | Round 8 |
| |
| | Fri, 4 December 2020 |
| | Benetton Treviso v Connacht | The Sportsground | 19:35 | 21:35 | Round 4 |
| | Gwent Dragons v Munster | Rodney Parade | 19:35 | 21:35 | Round 4 |
| |
| | Sat, 5 December 2020 |
| | Glasgow Warriors v Gwent Dragons | Scotstoun Stadium | 19:15 | 21:15 | Round 6 |
| |
| | Sat, 26 December 2020 |
| | Cardiff Blues v Gwent Dragons | Rodney Parade | 15:00 | 17:00 | Round 9 |
| | Ospreys v Scarlets | Parc y Scarlets | 17:15 | 19:15 | Round 9 |
| |
| | Sun, 27 December 2020 |
| | Connacht v Ulster | The Sportsground | 19:35 | 21:35 | Round 9 |
| |
| | Fri, 1 January 2021 |
| | Gwent Dragons v Scarlets | Parc y Scarlets | 17:15 | 19:15 | Round 10 |
| | Cardiff Blues v Ospreys | Cardiff Arms Park | 19:35 | 21:35 | Round 10 |
| |
| | Sat, 2 January 2021 |
| | Benetton Treviso v Zebre | Stadio Comunale Di Monigo | 13:00 | 15:00 | Round 10 |
| | Edinburgh v Glasgow Warriors | BT Murrayfield Stadium | 15:00 | 17:00 | Round 10 |
| | Munster v Ulster | Kingspan Stadium | 17:15 | 19:15 | Round 10 |
| | Connacht v Leinster | RDS Arena | 19:35 | 21:35 | Round 10 |
| |
| | Fri, 8 January 2021 |
| | Leinster v Ulster | RDS Arena | 19:35 | 21:35 | Round 11 |
| |
| | Sat, 9 January 2021 |
| | Benetton Treviso v Zebre | Stadio Sergio Lanfranchi | 13:00 | 15:00 | Round 9 |
| | Gwent Dragons v Ospreys | Rodney Parade | 17:15 | 19:15 | Round 11 |
| | Cardiff Blues v Scarlets | TBC | 19:35 | 21:35 | Round 11 |
| | Connacht v Munster | The Sportsground | 19:35 | 21:35 | Round 11 |
| |
| | Sat, 16 January 2021 |
| | Edinburgh v Glasgow Warriors | Scotstoun Stadium | 17:15 | 19:15 | Round 9 |
| |
| | Fri, 22 January 2021 |
| | Cardiff Blues v Scarlets | Parc y Scarlets | 20:00 | 22:00 | Round 14 |
| |
| | Sat, 23 January 2021 |
| | Edinburgh v Zebre | Stadio Sergio Lanfranchi | 13:00 | 15:00 | Round 11 |
| | Leinster v Munster | Thomond Park | 19:35 | 21:35 | Round 9 |
| |
| | Sun, 24 January 2021 |
| | Connacht v Ospreys | The Sportsground | 15:00 | 17:00 | Round 8 |
| |
| | Sat, 30 January 2021 |
| | Benetton Treviso v Munster | Stadio Comunale Di Monigo | 17:00 | 19:00 | Round 5 |
| | Leinster v Scarlets | Parc y Scarlets | 19:35 | 21:35 | Round 8 |
| |
| | Fri, 5 February 2021 |
| | Connacht v Gwent Dragons | Rodney Parade | 19:35 | 21:35 | Round 5 |
| |
| | Fri, 19 February 2021 |
| | Glasgow Warriors v Ulster | Scotstoun Stadium | 19:35 | 21:35 | Round 12 |
| | Gwent Dragons v Leinster | Rodney Parade | 19:35 | 21:35 | Round 12 |
| |
| | Sat, 20 February 2021 |
| | Benetton Treviso v Scarlets | Parc y Scarlets | 15:00 | 17:00 | Round 12 |
| | Ospreys v Zebre | Liberty Stadium | 17:15 | 19:15 | Round 12 |
| | Cardiff Blues v Connacht | The Sportsground | 19:35 | 21:35 | Round 12 |
| | Edinburgh v Munster | BT Murrayfield Stadium | 19:35 | 21:35 | Round 12 |
| |
| | Fri, 26 February 2021 |
| | Benetton Treviso v Connacht | Stadio Comunale Di Monigo | 17:30 | 19:30 | Round 13 |
| | Cardiff Blues v Munster | Cardiff Arms Park | 20:00 | 22:00 | Round 13 |
| | Ospreys v Ulster | Kingspan Stadium | 20:00 | 22:00 | Round 13 |
| |
| | Sat, 27 February 2021 |
| | Edinburgh v Scarlets | BT Murrayfield Stadium | 12:00 | 14:00 | Round 13 |
| | Gwent Dragons v Zebre | Stadio Sergio Lanfranchi | 17:15 | 19:15 | Round 13 |
| |
| | Sun, 28 February 2021 |
| | Glasgow Warriors v Leinster | RDS Arena | 17:30 | 19:30 | Round 13 |
| |
| | Fri, 5 March 2021 |
| | Connacht v Munster | Parc y Scarlets | 19:35 | 21:35 | Round 14 |
| |
| | Sat, 6 March 2021 |
| | Glasgow Warriors v Zebre | Stadio Sergio Lanfranchi | 13:00 | 15:00 | Round 14 |
| | Gwent Dragons v Ospreys | Bridgend RFC | 19:35 | 21:35 | Round 14 |
| | Leinster v Ulster | Kingspan Stadium | 19:35 | 21:35 | Round 14 |
| |
| | Sun, 7 March 2021 |
| | Benetton Treviso v Edinburgh | BT Murrayfield Stadium | 15:15 | 17:15 | Round 14 |
| |
| | Fri, 12 March 2021 |
| | Leinster v Zebre | Stadio Sergio Lanfranchi | 17:45 | 19:45 | Round 15 |
| | Glasgow Warriors v Ospreys | Scotstoun Stadium | 20:00 | 22:00 | Round 15 |
| | Munster v Scarlets | Thomond Park | 20:00 | 22:00 | Round 15 |
| |
| | Sat, 13 March 2021 |
| | Connacht v Edinburgh | The Sportsground | 19:35 | 21:35 | Round 15 |
| | Gwent Dragons v Ulster | Rodney Parade | 19:35 | 21:35 | Round 15 |
| |
| | Sun, 14 March 2021 |
| | Benetton Treviso v Cardiff Blues | Stadio Comunale Di Monigo | 13:00 | 15:00 | Round 15 |
| |
| | Fri, 19 March 2021 |
| | Benetton Treviso v Munster | Thomond Park | 18:00 | 20:00 | Round 16 |
| | Ulster v Zebre | Kingspan Stadium | 20:15 | 22:15 | Round 16 |
| | Leinster v Ospreys | RDS Arena | 20:15 | 22:15 | Round 16 |
| |
| | Sun, 21 March 2021 |
| | Glasgow Warriors v Gwent Dragons | Rodney Parade | 15:00 | 17:00 | Round 16 |
| |
| | Mon, 22 March 2021 |
| | Cardiff Blues v Edinburgh | Cardiff Arms Park | 20:00 | 22:00 | Round 16 |
| | Connacht v Scarlets | Parc y Scarlets | 20:00 | 22:00 | Round 16 |
| |
| | Sat, 27 March 2021 |
| | Benetton Treviso v Glasgow Warriors | Scotstoun Stadium | 11:45 | 13:45 | Round 11 |
| | Benetton Treviso v Glasgow Warriors | Scotstoun Stadium | 13:45 | 15:45 | Round 11 |
| | Leinster v Munster | RDS Arena | 17:00 | 19:00 | Round Final |
| |
| | Sun, 28 March 2021 |
| | Edinburgh v Gwent Dragons | Rodney Parade | 13:00 | 15:00 | Round 7 |
