Bisegni takes centre stage in Cardiff
Bisegni has won seven caps for his county, all of them coming on the wing, and his last appearance was against England in last year's Championship.
The in-form Zebre star's inclusion sees him join Tommaso Castello in midfield while the back three of Matteo Minozzi, Mattia Bellini and Tommaso Benvenuti remains unchanged.
Tommaso Allan and Marcello Violi are the halfback pairing still while up front Sergio Parisse captains the side from No.8 in between flankers Seb Negri and Maxime Mbanda.
The second row consists of Alessandro Zanni and Dean Budd while Leonardo Ghiraldini is at hooker with Andrea Lovotti and Simone Ferrari the props.
On the bench there is a return for Giovanni Licata with George Biagi injured while Guglielmo Palazzani is the scrumhalf cover with Edoardo Gori injured. The final change sees Oliviero Fabiani come in as the replacement hooker.
Italy: 15 Matteo Minozzi, 14 Tommaso Benvenuti, 13 Giulio Bisegni, 12 Tommaso Castello, 11 Mattia Bellini, 10 Tommaso Allan, 9 Marcello Violi, 8 Sergio Parisse, 7 Maxime Mbanda, 6 Sebastian Negri, 5 Dean Budd, 4 Alessandro Zanni, 3 Simone Ferrari, 2 Leonardo Ghiraldini, 1 Andrea Lovotti.
Replacements: 16 Oliviero Fabiani, 17 Nicola Quaglio, 18 Tiziani Pasquali, 19 Federico Ruzza, 20 Giovanni Licata, 21 Guglielmo Palazzani, 22 Carlo Canna, 23 Jayden Hayward.
Date: Sunday, March 11
Venue: Principality Stadium, Cardiff
Kick-off: 15.00 (15.00 GMT, 16.00 Italy time)
Referee: Jérôme Garcès (France)
Assistant referees: Pascal Gaüzère (France), Andrew Brace (Ireland)
TMO: Marius Jonker (South Africa)