Tupou earns first start for Rebels
Michael Ruru will return to the starting line-up for the injured Will Genia.
Tom English returns to the centres in place of Billy Meakes.
The Rebels travel to the nation's capital having already defeated the Brumbies 33-10 in Round Four at AAMI Park, and will be aiming to improve on their overall record at GIO Stadium – Melbourne having won there only once from five attempts.
Melbourne Rebels: 15 Jack Maddocks, 14 Semisi Tupou, 13 Tom English, 12 Reece Hodge, 11 Marika Koroibete, 10 Jack Debreczeni, 9 Michael Ruru, 8 Amanaki Mafi, 7 Colby Fainga'a, 6 Angus Cottrell, 5 Adam Coleman (captain), 4 Geoff Parling, 3 Sam Talakai, 2 Anaru Rangi, 1 Fereti Sa'aga,
Replacements: 16 Nathan Charles, 17 Tetera Faulkner, 18 Jermaine Ainsley, 19 Matt Philip, 20 Sam Jeffries, 21 Lopeti Timani, 22 Harrison Goddard, 23 Billy Meakes.
Date: Saturday, May 12
Venue: GIO Stadium, Canberra
Kick-off: 19.45 (09.45 GMT)
Referee: Nic Berry (Australia)
Assistant referees: Will Houston (Australia), Jordan Way (Australia)
TMO: Damien Mitchelmore (Australia)