Hughes at No.8 for England
Joe Launchbury (Wasps) will play in his 50th game for England having made his debut for his country in 2012.
Captain Dylan Hartley will become England's second most capped player of all time when he steps out for the 92nd time on Saturday. Hartley will surpass Jonny Wilkinson on 91 caps and now only sits behind Jason Leonard (114).
"We are really excited about this game and we will need to be very tough and relentless in our defence to keep the pressure on this Scottish team," said Jones.
"They are renowned for their attack so we will need to put pressure on them, create opportunities through our defence for our attack, and convert those opportunities to points.
"Our preparation over the last two weeks has been important in that it has allowed us to regenerate the players, improve the fitness of some and to get a clear understanding on how we will play this weekend.
"Nathan Hughes has worked hard on his fitness. I've never seen him in better physical condition so we are looking forward to him playing. Nathan has a key role in defence and in us gaining momentum in attack."
Jones added on Launchbury's milestone: "For any player to play 50 caps for their country is a wonderful achievement. Joe has worked really hard to improve and is such an important player for us."
Joe Marler (Harlequins) is named as a finisher following his suspension.
England: 15 Mike Brown, 14 Anthony Watson, 13 Jonathan Joseph, 12 Owen Farrell, 11 Jonny May, 10 George Ford, 9 Danny Care, 8 Nathan Hughes, 7 Chris Robshaw, 6 Courtney Lawes, 5 Maro Itoje, 4 Joe Launchbury, 3 Dan Cole, 2 Dylan Hartley (captain), 1 Mako Vunipola.
Replacements: 16 Jamie George, 17 Joe Marler, 18 Harry Williams, 19 George Kruis, 20 Sam Underhill, 21 Richard Wigglesworth, 22 Ben Te'o, 23 Jack Nowell.
Date: Friday, Febraury 23
Venue: Murrayfield Stadium, Edinburgh
Kick-off: 16.45 (16.45 GMT, 16.45 England time)
Referee: Nigel Owens (Wales)
Assistant referees: Jérôme Garcès (France), Andrew Brace (Ireland)
TMO: Simon McDowell (Ireland)
