Alberts blow for Boks
Alberts injured his calf during the captain's run on Friday.
Pieter-Steph du Toit will start in the No.7 jersey, with his place on the bench going to Eben Etzebeth.
"Willem felt some discomfort during the captain's run and we took him out of the session as a precautionary measure," said Springbok team doctor Craig Roberts.
"We reassessed him again this [Saturday] morning and decided it would be better to withdraw him from the team."
As a result of Alberts' withdrawal, the starting team now only has a combined total of 851 Test caps.
It is still more than the previous most experienced Springbok side (836 caps), which played in South Africa's last Rugby World Cup match against Australia in Wellington, New Zealand in 2011.
South Africa – revised: 15 Zane Kirchner, 14 Bryan Habana, 13 Jesse Kriel, 12 Jean de Villiers (captain), 11 Lwazi Mvovo, 10 Pat Lambie, 9 Ruan Pienaar, 8 Schalk Burger, 7 Pieter-Steph du Toit, 6 Francois Louw, 5 Victor Matfield, 4 Lood de Jager, 3 Jannie du Plessis, 2 Bismarck du Plessis, 1 Tendai Mtawarira.
Replacements: 16 Adriaan Strauss, 17 Trevor Nyakane, 18 Coenie Oosthuizen, 19 Eben Etzebeth, 20 Siya Kolisi, 21 Fourie du Preez, 22 Handré Pollard, 23 JP Pietersen.
Date: Saturday, September 19
Venue: Brighton Community Stadium, Brighton
Kick-off: 16.45 (17.45 SA time; 15.45 GMT; 00.45 Sunday, September 20, Japan time)
Referee: Jérôme Garcès (France)
Assistant referees: JP Doyle (England), Federico Anselmi (Argentina)
TMO: Graham Hughes (England)