Armand to the rescue for England
Hughes limped out of the first half of the 16-22 reverse at the Stade de France, while England have also now confirmed a knock for Lawes, with neither included in a 32-man selection.
Both forwards are set for scans on Monday, with Exeter Chiefs' Don Armand called up.
Captain Dylan Hartley missed the Paris loss with a tight calf, but he is included as England look to deny Ireland a Grand Slam at Twickenham.
Sam Simmonds, on the bench against France, is not in the squad preparing for Saturday's match, when the hosts will be out to avoid a third consecutive loss.
It is a game that also affords Eddie Jones' side the chance to gain some revenge for last year's Six Nations reverse against Ireland, which denied England a second Grand Slam on the bounce.
Backs: Mike Brown (Harlequins), Danny Care (Harlequins), Elliot Daly (Wasps), Nathan Earle (Saracens), Owen Farrell (Saracens), George Ford (Leicester Tigers), Jonathan Joseph (Bath), Alex Lozowski (Saracens), Jonny May (Leicester Tigers), Henry Slade (Exeter Chiefs), Marcus Smith (Harlequins), Denny Solomona (Sale Sharks), Ben Te'o (Worcester Warriors), Anthony Watson (Bath), Richard Wigglesworth (Saracens).
Forwards: Don Armand (Exeter Chiefs), Dan Cole (Leicester Tigers), Luke Cowan-Dickie (Exeter Chiefs), Charlie Ewels (Bath), Jamie George (Saracens), Dylan Hartley, (Northampton Saints), James Haskell (Wasps), Alec Hepburn (Exeter Chiefs), Nick Isiekwe (Saracens), Maro Itoje (Saracens), George Kruis (Saracens), Joe Launchbury (Wasps), Joe Marler (Harlequins), Chris Robshaw (Harlequins), Sam Simmonds (Exeter Chiefs), Kyle Sinckler (Harlequins), Mako Vunipola (Saracens).